The NADPH oxidase inhibitors iodonium diphenyl and cadmium sulphate inhibit hypoxic pulmonary vasoconstriction in isolated rat pulmonary arteries

Title:

The NADPH oxidase inhibitors iodonium diphenyl and cadmium sulphate inhibit hypoxic pulmonary vasoconstriction in isolated rat pulmonary arteries

Creator:

Jones, R. D., Thompson, J. S., and Alyn H. Morice

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e9f8a2d2-8e71-41b1-9261-037ff3d649cc
uuid:e9f8a2d2-8e71-41b1-9261-037ff3d649cc
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, NADPH oxidase, hypoxic pulmonary vasoconstriction, iodonium diphenyl cadmium sulphate, isolated rat pulmonary artery, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Interest surrounds the role of an NADPH oxidase-like enzyme in hypoxic pulmonary vasoconstriction (HPV). We have studied the effects of the NADPH oxidase inhibitors iodonium diphenyl (ID) and cadmium sulphate (CdSO4) upon HPV of isolated rat pulmonary arteries (n = 73, internal diameter 545± 23 mm). Vessels were preconstricted with prostaglandin F2a (PGF2a, 0.5 or 5 mM) prior to a hypoxic challenge. ID (10 or 50 mM), CdSO4 (100 mM) or vehicle (50 ml) was added for 30 min before re-exposure to PGF2a and hypoxia. ID and CdSO4 significantly inhibited HPV. In vessels preconstricted with 5 mM PGF2a, ID (10 and 50 mM) reduced HPV from 37.4± 5.6 % to 9.67± 4.4 % of the contractile response elicited by 80 mM KCl (P<0.05) and from 30.1± 5.0 % to 0.63± 0.6% 80 mM KCl response (P<0.01), respectively. CdSO4 (100 mM) reduced HPV from 29.4±4.0 % to 17.1±2.2% 80 mM KCl response (P<0.05). In vessels preconstricted with 0.5 mM PGF2a, ID (10 and 50 mM) reduced HPV from 16.0± 3.15% to 3.36± 1.44 % 80 mM KCl response (P<0.01) and from 15.0± 1.67 % to 2.82± 1.40 % 80 mM KCl response (P<0.001), respectively. Constriction to PGF2a was potentiated by ID. ID and CdSO4, at concentrations previously shown to inhibit neutrophil NADPH oxidase, attenuate HPV in isolated rat pulmonary arteries. This suggests that an NADPH oxidase-like enzyme is involved in HPV and could act as the pulmonary oxygen sensor., R. D. Jones, J. S. Thompson, A. H. Morice., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2000 Volume:49 | Number:5

Harvested from:

CDK

Metadata only:

false