Correlation between [Na+], [glycosaminoglycan] and Na+/K+ pump density in the extracellular matrix of bovine articular cartilage

Title:

Correlation between [Na+], [glycosaminoglycan] and Na+/K+ pump density in the extracellular matrix of bovine articular cartilage

Creator:

Mobasheri, A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e74a6d76-82c6-49be-9026-d29b42463ca7
uuid:e74a6d76-82c6-49be-9026-d29b42463ca7

Subject:

cartilage, chondrocyte, glycosaminoglycan, extracellular [Na+], and Na+ /K+ pump density

Type:

article, model:article, and TEXT

Description:

The plasma membrane abundance of the Na + /K+ pump or Na + ,K+-ATPase depends on the intracellular concentration of Na+ in almost all animal cells. In cartilage, chondrocytes are surrounded by an extracellular matrix which consists of collagen and proteoglycan, a ground substance composed of glycosaminoglycan (GAG) side chains with a high fixed negative charge density. The polyanionic nature of the GAGs tends to draw monovalent cations into the matrix resulting in high [Na + ] which may exceed 250 mM. As the concentration of GAGs in the tissue increases, so does [Na+]. In this study, it was found that the density of the Na+/K+ pump, measured by 3H-ouabain binding, correlates with the concentration of GAGs in the tissue. This indicates that chondrocytes are sensitive to their ionic environment and respond to local [Na + ] variations by regulating the abundance of the Na + /K+ pump.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1998 Volume:47 | Number:1

Harvested from:

CDK

Metadata only:

false