Evidences of apoptosis during the early phases of soleus muscle atrophy in hindlimb suspended mice

Title:

Evidences of apoptosis during the early phases of soleus muscle atrophy in hindlimb suspended mice

Creator:

Fabiane Mendonça Ferreira, Neuparth, M. J., Vittorino, R., Appell, H. J., Amado, F., and Duarte, J. A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e53622d5-08a7-4988-8639-8dffc11b6d24
uuid:e53622d5-08a7-4988-8639-8dffc11b6d24
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, mitochondrie, buněčná smrt, svalová atrofie, physiology, mitochondrias, cell death, muscular atrophy, unloading, muscle ultrastructure, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The purpose of this study was to investigate the occurrence and time-course of apoptosis in soleus skeletal muscle during the first 48 hours of unloading. Fifty Charles River mice were randomly divided into five groups (n=10 each) according to the time of hindlimb suspension (HS). Mice we re suspended for 0 (Control), 6 (6HS), 12 (12HS), 24 (24HS), and 48 hours (48HS). Soleus muscle atrophy was confirmed by a significant decrease of 20 % in muscle-wet weight and of 5 % in the ratio protein concentration/muscle wet-weight observed after 48 hours of unloading. The apoptotic index, the AIF (apoptosis-inducing factor) and p53 expression presented th eir uppermost value (304 %, 241 % and 246 %, respectively) at 24HS, and were preceded by the highest activity of caspase-3 and -8 at 12HS (170 % and 218 %, respectively) and of Bax/Bcl-2 content at 6HS (160 %). There were no marked ultrastructural alterations until 24 hours of simulated weightlessness. Lysosomal autophagic activity and infiltration of phagocytic cells were observed at 24HS and 48HS and might have contributed to the degenerative changes noticed in both groups. Though not consistently supported by morphological evidences, the biochemical parameters sustain the concept that the occurrence of apoptosis parallels the soleus atrophic response in its early phase., R. Ferreira, M. J. Neuparth, R. Vittorino, H. J. Appell, F. Amado, J. A. Duarte., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:4

Harvested from:

CDK

Metadata only:

false