Environmental regulation of the purine synthesis enzyme purH transcript during adult diapause in Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Title:

Environmental regulation of the purine synthesis enzyme purH transcript during adult diapause in Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Creator:

Yocum, George D.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e130b042-ab1b-4446-bb98-32cfacef115d
uuid:e130b042-ab1b-4446-bb98-32cfacef115d

Subject:

Diapause, Colorado potato beetle, gene expression, and purine

Type:

article, model:article, and TEXT

Description:

A 1962 bp cDNA clone of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (purH) was isolated from diapausing adults of Leptinotarsa decemlineata using RT-PCR and 3' and 5'-RACE. The probe generated from this clone hybridized to a transcript approximately 1960 bp in length on northern blots. The clone encodes for a deduced protein 594 amino acids in length with 73% identity, 83% similarity to purH from Drosophila melanogaster. Northern blot (total RNA) analysis determined that L. decemlineata purH (LdpurH) was downregulated in diapausing beetles stored at 10°C. Developmental studies revealed that LdpurH is expressed at nearly constant high levels in both nondiapausing and prediapause adults. LdpurH expression during the first 20 days of diapause is equivalent to that seen in nondiapausing beetles, after which expression decreases. Exposing 70 day old diapausing beetles to 20°C for 24 h induced an increase in expression of purH indicating that purH is regulated by temperature in diapausing/overwintering beetles.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2004 Volume:101 | Number:2

Harvested from:

CDK

Metadata only:

false