Evaluation of molecular changes of distal organs after small bowel transplantation

Title:

Evaluation of molecular changes of distal organs after small bowel transplantation

Creator:

Pavel Urban, Rabajdová, M., Feterik, Š., Bódy, G., Granda, T., Mária Mareková, and Veselá, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:dac1762a-b597-445b-8000-54e89a160be7
uuid:dac1762a-b597-445b-8000-54e89a160be7
issn:0862-8408

Subject:

apoptóza, cytokiny, genová exprese, apoptosis, cytokines, gene expression, inflammation mediators, molecular chaperones, 14, and 612

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

The ischemia and reperfusion of a jejunal graft during transplantation triggers the stress of endoplasmic reticulum thus inducing the synthesis of pro-inflammatory cytokines. Spreading of these signals stimulate immunological reactions in distal tissues, i.e. lung, liver and spleen. The aim of this study was to detect the molecular changes in liver and spleen induced by transplanted jejunal graft with one or six hours of reperfusion (group Tx1 and Tx6). Analysis of gene expression changes of inflammatory mediators (TNF-α, IL-10) and specific chaperones (Gadd153, Grp78) derived from endoplasmic reticulum (ER) was done and compared to control group. The qRT-PCR method was used for amplification of the specific genes. The levels of corresponding proteins were detected by Western blot with immunodetection. Protein TNF-α was in liver tissue significantly overexpressed in the experimental group Tx1 by 48 % (p<0.001). In the group Tx6 we found decreased levels of the same protein to the level of controls. However, the protein concentrations of TNF-α in spleen showed increased levels in group Tx1 by 31 % (p<0.001) but even higher levels in the group Tx6 by 115 % (p<0.001) in comparing to controls. Our data demonstrated that the spleen is more sensitive to posttransplantation inflammation than liver, with consequent stress of ER potentially inducing apoptosis and failure of basic functions of lymphoid tissue., P. Urban, M. Rabajdová, Š. Feterik, G. Bódy, T. Granda, M. Mareková, J. Veselá., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2018 Volume:67 | Number:4

Harvested from:

CDK

Metadata only:

false