Phospholemman does not participate in forskolin-induced swine carotid artery relaxation

Title:

Phospholemman does not participate in forskolin-induced swine carotid artery relaxation

Creator:

Meeks, M. K., Han, S., Tucker, Amy L., and Rembold, Christopher M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:d7be5b17-f491-480d-a06e-b68d32a6ee47
uuid:d7be5b17-f491-480d-a06e-b68d32a6ee47
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, kardiovaskulární fyziologie, hladké svaly, bílkoviny, cardiovascular physiology, smooth muscles, proteins, cyclic AMP (adenosine monophosphate), FXYD protein, HSP20, myosin light chain, vascular smooth muscle, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Phosphorylation of phospholemman (PLM) on ser68 has been proposed to at least partially mediate cyclic AMP (cAMP) mediated relaxation of arterial smooth muscle. We evaluated the time course of the phosphorylation of phospholemman (PLM) on ser68, myosin regulatory light chains (MRLC) on ser19, and heat shock protein 20 (HSP20) on ser16 during a transient forskolin-induced relaxation of histamine-stimulated swine carotid artery. We also evaluated the dose response for forskolin- and nitroglycerin-induced relaxation in phenylephrine-stimulated PLM-/- and PLM+/+ mice. The time course for changes in ser19 MRLC dephosphorylation and ser16 HSP20 phosphorylation was appropriate to explain the forskolin-induced relaxation and the recontraction observed upon washout of forskolin. However, the time course for changes in ser68 PLM phosphorylation was too slow to explain forskolin-induced changes in force. There was no difference in the phenylephrine contractile dose response or in forskolin-induced relaxation dose response observed in PLM-/- and PLM+/+ aortae. In aortae precontracted with phenylephrine, nitroglycerin induced a slightly, but significantly greater relaxation in PLM-/- compared to PLM+/+ aortae. These data are consistent with the hypothesis that ser19 MRLC dephosphorylation and ser16 HSP20 phosphorylation are involved in forskolin-induced relaxation. Our data sugge st that PLM phosphorylation is not significantly involved in forskolin-induced arterial relaxation., M. K. Meeks, S. Han, A, L. Tucker, C. M. Rembold., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:5

Harvested from:

CDK

Metadata only:

false