CD27+ peripheral blood b-cells are a useful biodosimetric marker in vitro

Title:

CD27+ peripheral blood b-cells are a useful biodosimetric marker in vitro

Creator:

Zuzana Řeháková, Šinkora, J., Marcela Vlková, Doris Vokurková, Jan Österreicher, Jaroslava Vávrová, and Daniel Driák

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:d3d071af-2ded-44df-96c7-d14ea8a34f05
uuid:d3d071af-2ded-44df-96c7-d14ea8a34f05
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, klinická imunologie, radiobiologie, dozi, clinical immunology, radiobiology, B-cells, CD27+, biodosimetric marker, γ-irradiation, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The CD8+ natural killer (NK) subpopulation has recently been identified as a fast and reliable biodosimetric indicator within human peripheral blood mononuclear cells (PBMC) in vitro. In irradiated and subsequently cultivated PBMC, a decrease of the relative number of intact CD3-CD8+ lymphocytes 16 and 48 h after treatment has allowed for estimating the received dose in the range of 0 - 10 Gy and lethal/sublethal dose discrimination, respectively. Here we show that suitable biodosimeters can also be found in the peripheral blood B-cell compartment. Multiparameter flow cytometric analysis of irradiated and subsequently cultivated human PBMC revealed that both the CD27+ and CD21- B-cell subpopulations can be used as biodosimeters and the CD19+CD27+ lymphocytes have proved useful for retrospective determination of the received dose in the range of 0 - 6 Gy. In addition, several CD19+ lymphocyte subsets characterized by co-expression of CD21, CD27 and CD38 have been shown to bear biodosimetric potential, too. However, when important parameters like the original size within the CD19+ compartment, its radiation-induced changes and data variation had been taken into account, the CD27+ subpopulation proved superior to the other B-cell subpopulations and subsets. It appears that, in the dose range of 0 - 6 Gy, the relative decrease of CD27+ B lymphocytes provides more sensitive and reliable data than that of CD8+ NK-cells due mainly to lower data variation. In contrast to CD27+ B-cells, the proportions of CD27+ subpopulations of T-cells were not affected by irradiation. We have also proposed a simple experimental protocol based on full blood cultivation and three-color CD27/CD3/CD19 immuno-phenotyping as a time-saving and inexpensive approach for practical biodosimetric evaluations on simple, three-to-four color flow cytometers., Z. Řeháková, J. Šinkora, M. Vlková, D. Vokurková, J. Österreicher, J. Vávrová, D. Driák., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:4

Harvested from:

CDK

Metadata only:

false