An evidence for regulatory cross-talk between aryl hydrocarbon receptor and glucocorticoid receptor in HepG2 cells

Title:

An evidence for regulatory cross-talk between aryl hydrocarbon receptor and glucocorticoid receptor in HepG2 cells

Creator:

Zdeněk Dvořák, Radim Vrzal, Petr Pávek, and Jitka Ulrichová

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:d34565b6-c685-4409-b510-a6cabcc31271
uuid:d34565b6-c685-4409-b510-a6cabcc31271
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, biochemie, buněčné signály, membránové receptory, xenobiotika, glukokortikoidy, genetická informace, biochemistry, cell signals, membrane receptors, xenobiotics, glucocorticoids, genetic information, dioxin, transcriptional control, 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Aryl hydrocarbon receptor (AhR) and glucocorticoid receptor (GR) play crucial role in the regulation of drug metabolizing enzymes and in many essential physiological processes. Cellular signaling by these receptors shares several functional and regulatory features. Here we investigated regulatory cross-talk between these two receptors. Human hepatoma cells (HepG2) were the model of choice. We analyzed the effects of dexamethasone (DEX) and dioxin (TCDD) on i) expression of AhR and GRα mRNAs; ii) levels of AhR and GR proteins; iii) transcriptional activities of AhR and GR in reporter assays; iv) 7-ethoxyresorufin-O-deethylase activity (EROD). We found that both DEX and TCDD affected AhR and GR mRNAs expression, proteins levels and transcriptional activities in HepG2 cells. These effects on cellular signaling by AhR and GR comprised up-/down-regulation of gene expression and ligand-dependent protein degradation. We conclude that interactive regulatory cross-talk between GR and AhR receptors in HepG2 cells defines possible implications in physiology and drug metabolism. Future research should be focused on the investigation of AhR-GR cross-talk in various normal human cells and tissues both in vitro and in vivo., Z. Dvořák, R. Vrzal, P. Pávek, J. Ulrichová., and Obsahuje bibliografii a bibliigrafické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:3

Harvested from:

CDK

Metadata only:

false