Irradiation-induced in vivo re-localization of NADPH-protochlorophyllide oxidoreductase from prolamellar body to stroma of barley etioplast

Title:

Irradiation-induced in vivo re-localization of NADPH-protochlorophyllide oxidoreductase from prolamellar body to stroma of barley etioplast

Creator:

Kovacevic, D., Dewez, D., and Popovic, D.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:cfa37fe5-58d6-4198-b2d6-922ff002ab30
uuid:cfa37fe5-58d6-4198-b2d6-922ff002ab30
issn:0300-3604
doi:10.1007/s11099-007-0016-2

Subject:

chlorophyll(ide), chloroplast development, envelope, fluorescence spectra, Hordeum vulgare, and prothylakoid

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Distribution of NADPH-protochlorophyllide oxidoreductase (POR) in etioplast of etiolated barley leaf was studied by using Western blot analyses of etioplast fractions isolated on a sucrose gradient. When the leaf was exposed to light, POR content decreased in the etioplast inner membrane and prolamellar body sub-membrane fraction while it was simultaneously increased in the stroma. By using 77 K fluorescence spectroscopy analyzes, we found for irradiated etiolated leaf that the POR protein in the stroma was co-localized with chlorophyllide (Chlide) emitting at 678 nm. Relocalization of the POR-Chlide complex induced by irradiation suggests that POR participates in the pigment transport processes during early stages of the thylakoid membrane development. and D. Kovacevic, D. Dewez, R. Popovic.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

105-109

Source:

Photosynthetica | 2007 Volume:45 | Number:1

Harvested from:

CDK

Metadata only:

false