Comparative studies on carbohydrates of several myxosporcan parasites of fish using lectin histochemical methods

Title:

Comparative studies on carbohydrates of several myxosporcan parasites of fish using lectin histochemical methods

Creator:

Muňoz, Pilar, Palenzuela, Oswaldo, Alvarez-Pellitero, Pilar, and Sitja-Bobadilla, Ariadna

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c896f6e0-73df-4368-9100-b37c430303b6
uuid:c896f6e0-73df-4368-9100-b37c430303b6
urnnbn:urn:nbn:cz:aba007-000ds5

Subject:

Myxozoa, parasites, lectins, histochemistry, Teleostei, and carbohydrates

Type:

model:internalpart and TEXT

Format:

print

Description:

A histochemical study using lectin methods was performed on myxosporean parasites from vastly different fish hosts from marine and fresh waters. Six biotinylated lectins were used (WGA, SBA, BS-I, Con-А, UEA-I and SNA). The binding paltem of Con-A and WGA revealed the presence of mannose and/or glucose, and N-acetyl-D-glucosamine respectively, in polar capsules and valves of most of the myxosporea assayed. Thus, chitin may be present in polar capsules and/or valves of myxosporean spores. The BS-I binding pattern showed the presence of a-!)-galactose and/or N-acetyl-D-galactosamine residues in polar capsules of Kudoa sp., Zschokkeìla mugilis Sitjà-Bobadilla et Alvarez-Pellitero, 1993 and Leplotheca sp., and in the valves of the latter. Scarce amounts of N-acetyl-D-galactosamine and/or α-D-galactose were demonstrated by SBA binding in Sphaerospora dicentrarchi Sitjà-Bobadilla et Alvarez-Pellitero 1992, Leplotheca sp. and Kudoa sp. valves, and in Leptotheca sp. polar capsules. The UEA-I staining indicated the absence ofa-L-fucose in all the myxosporea assayed except in Leptotheca sp. N-acety!neuraminic acid was detected with SNA in the polar capsules and sporoplasms of Polysporoptasma sparis Sitjà-Bobadilla et-Alvarez-Pellitero, 1995 and in the polar capsules and valves of Kudoa sp. These results indicate that, although Myxosporea may have conserved carbohydrate structures, some of them can show significantly different binding patterns, which may be useful in diagnostic and functional studies.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

241-247 and 3-9

Source:

Folia parasitologica | 1999 Volume:46 | Number:4

Harvested from:

CDK

Metadata only:

false