A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity

Title:

A serine protease from the midgut of the bumblebee, Bombus ignitus (Hymenoptera: Apidae): cDNA cloning, gene structure, expression and enzyme activity

Creator:

Choo, Young Moo, Lee, Kwang Sik, Yoon, Hyung Joo, Lee, Sang Beom, Kim, Ji Hyeon, Sohn, Hung Dae, and Jin, Byung Rae

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c7d32e4c-9f45-45ec-8f7c-d7e820920c7c
uuid:c7d32e4c-9f45-45ec-8f7c-d7e820920c7c

Subject:

Apidae, baculovirus expression vector, Bombus ignitus, bumblebee, cDNA cloning, enzyme, expression, gene structure, and serine protease

Type:

article, model:article, and TEXT

Description:

A serine protease gene was cloned from the bumblebee, Bombus ignitus. The B. ignitus serine protease (BiSP) gene spans 1702 bp and consists of four introns and five exons coding for 250 amino acid residues. Southern blot analysis of genomic DNA suggested that BiSP gene is a single copy gene. The cDNA encoding BiSP was expressed as a 28-kDa polypeptide in baculovirus-infected insect cells and the recombinant BiSP showed activity in a protease enzyme assay. BiSP was specifically expressed in the midgut of B. ignitus queens, males, and workers, suggesting that the BiSP is a gut enzyme involved in the digestion of dietary proteins.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2007 Volume:104 | Number:1

Harvested from:

CDK

Metadata only:

false