Effect of High Glucose Concentrations on Expression of ELAM 1, VCAM-1 and ICAM-1 in HUVEC with and without Cytokine Activation

Title:

Effect of High Glucose Concentrations on Expression of ELAM 1, VCAM-1 and ICAM-1 in HUVEC with and without Cytokine Activation

Creator:

Altannavch, Ts., Roubalová, K., Kučera, P., and Anděl, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c4167d91-ea2b-4e9a-be48-8fadb2c7429e
uuid:c4167d91-ea2b-4e9a-be48-8fadb2c7429e

Subject:

Adhesion molecules, Atherosclerosis, Endothelial cells, Diabetes mellitus, and High glucose

Type:

article, model:article, and TEXT

Description:

Diabetes mellitus is associated with an increased prevalence of endothelial dysfunction and development of atherosclerotic vascular diseases. We demonstrate here that hyperglycemia results in the expression of adhesion molecules on endothelial cells in vitro. Incubation of human umbilical vein endothelial cells (HUVEC) in a culture medium with 11.0 mM, 16.5 mM and 22.0 mM glucose concentrations induced the expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and endothelial-leukocyte adhesion molecule-1 (ELAM-1). This effect was detectable after 24 h incubation of HUVEC with a high glucose concentration. The effect of high glucose concentration on TNF- induced expression of ELAM-1, VCAM-1 and ICAM-1 was negligible, if at all. These results show that even a short-term exposure of endothelial cells (ECs) to high glucose concentration leads to their activation associated with increased expression of adhesion molecules such as ELAM-1, VCAM-1 and ICAM-1.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2004 Volume:53 | Number:1

Harvested from:

CDK

Metadata only:

false