The influence of testosterone on the expression and function of vitamin D3 receptor (VDR) protein in the porcine ovarian follicle

Title:

The influence of testosterone on the expression and function of vitamin D3 receptor (VDR) protein in the porcine ovarian follicle

Creator:

Herian, M., Luck, M. R., and Grzesiak, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c342b197-ce34-412b-af8e-11b17afe8ccb
uuid:c342b197-ce34-412b-af8e-11b17afe8ccb
issn:0862-8408

Subject:

prasata, pigs, androgens, vitamin D3 receptor, ovarian follicle, 14, and 612

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

Recently it has been shown that vitamin D3 acting via its cognate receptor (VDR) regulates the growth, differentiation and function of female reproductive tissues including ovary. The aim of the study was to examine the effect of testosterone (T) and its antagonist 2-hydroxyflutamide (HF) on VDR protein expression and function in porcine ovarian follicles. Medium size antral follicles expressing great amount of androgen receptors and represent high steroidogenic activity were used in this research. After 6 h incubation of whole follicles with T, HF or T+HF, immunohistochemical analysis of VDR revealed its nuclear localization in granulosa and theca interna cells in control and experimental groups. The expression of VDR protein was shown as a band of 48 kDa. There were no significant differences between either experimental group and the control. T influenced the function of VDR through decreased formation of VDR/RXR (retinoid X receptor) complexes (P<0.05) in both granulosa and theca interna cells, but HF abolished this effect only in granulosa cells (P<0.05). These results suggest that androgens regulate the response of follicular cells to vitamin D3 in pigs ovary via regulation of VDR transcriptional activity., M. Herian, M. R. Luck, M. Grzesiak., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2018 Volume:67 | Number:3

Harvested from:

CDK

Metadata only:

false