Increase of intracellular BAFF in B cells of Sjögren's patients is not affected by decrease of BAFFR

Title:

Increase of intracellular BAFF in B cells of Sjögren's patients is not affected by decrease of BAFFR
Increase of intracellular BAFF in B cells of Sjögren's patients is not affected by decrease of BAFFR

Creator:

Krejsek, Jan, Koláčková, Martina, Lindrová, I., Slezák, Radovan, and Andrýs, Ctirad

Contributor:

Krejsek, Jan , 1958-, Koláčková, Martina, Lindrová, I., Slezák, Radovan , 1958-, and Andrýs, Ctirad , 1962-

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bmc15040792-7af3a6ae-57df-4675-b3f6-8a6e58b19841
uuid:bmc15040792-7af3a6ae-57df-4675-b3f6-8a6e58b19841
local:bmc15040792
http://actamedica.lfhk.cuni.cz/
doi: 10.14712/18059694.2015.88
local: bmc15040792

Subject:

faktor aktivující B-buňky--biosyntéza--krev, receptor faktoru aktivujícího B-buňky--biosyntéza, B-lymfocyty--metabolismus, ženské pohlaví, lidé, intracelulární tekutina, intracelulární membrány, mužské pohlaví, lidé středního věku, monocyty--metabolismus, and Sjoegrenův syndrom--metabolismus

Type:

model:article, article, Text, časopisecké články, práce podpořená grantem, and TEXT

Format:

print, text, and regular print

Description:

The presence of a broad spectrum of autoantibodies in Sjögren's syndrome (SjS) patients is the result of abnormal B-cell regulation that can be at least partially explained by abnormal BAFF/BAFFR regulation. The objective of this study was to determine both membrane and intracellular expression of BAFF/BAFFR in monocytes and B-cells in peripheral blood of 19 primary Sjögren's syndrome patients and 20 healthy controls using flow cytometry. We also measured sBAFF in serum. Compared to healthy controls, both surface and intracellular expression of BAFF was significantly increased in monocytes and B-cells of SjS patients. Also serum sBAFF level was elevated. Expression of BAFFR on B-cells of SjS patients was surprisingly decreased, but there was no clear increase or decrease within monocytes. Our results indicate that activated monocytes communicate with B-cells via BAFF and BAFFR, so that B-cells are stimulated, but BAFF is also produced to stimulate cells in autocrine way. The decrease of BAFFR expression in SjS patients suggests that there is the mechanism that attempts to take over in order to balance the high level of BAFF. and J. Krejsek, M. Koláčková, I. Lindrová, R. Slezák, C. Andrýs

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Relation:

Acta medica (Hradec Králové) Universitas Carolina, Facultas Medica Hradec Králové--MED00010947

Source:

Acta medica (Hradec Králové) | 2015 Volume:58 | Number:1

Harvested from:

CDK

Metadata only:

false

Date:

2015