Antioxidative effect of epigallocatechin gallate against D-galactosamine-induced injury in primary culture of rat hepatocytes

Title:

Antioxidative effect of epigallocatechin gallate against D-galactosamine-induced injury in primary culture of rat hepatocytes
Antioxidative effect of epigallocatechin gallate against D-galactosamine-induced injury in primary culture of rat hepatocytes

Creator:

Moravcová, Alena, Červinková, Zuzana, Kučera, Otto, Mezera, Vojtěch, and Lotková, Halka

Contributor:

Moravcová, Alena, Červinková, Zuzana , 1951-, Kučera, Otto , 1977-, Mezera, Vojtěch, and Lotková, Halka , 1967-

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bmc14071296-90c388fe-cbf5-495f-b708-74f42ced2eb1
uuid:bmc14071296-90c388fe-cbf5-495f-b708-74f42ced2eb1
local:bmc14071296
https://www.lfhk.cuni.cz/Faculty/Publications/Acta-Medica/Archive/2014/Acta-Medica-2014-Vol-57-No-1/
local: bmc14071296

Subject:

zvířata, antioxidancia--farmakologie, katechin--analogy a deriváty--farmakologie, buněčné kultury, galaktosamin--farmakologie, glutathion--farmakologie, hepatocyty--účinky léků--patologie, and krysy

Type:

model:article, article, Text, časopisecké články, práce podpořená grantem, and TEXT

Format:

print, text, and regular print

Description:

Literature data support that green tea and its major component epigallocatechin gallate (EGCG) have powerful antioxidant effects. Contrary, hepatotoxicity can be induced by high-dose EGCG. The timing of exposure to green tea in relation to administration of hepatotoxic agent plays an import role too. The aim of our work was a verification of antioxidative effect of EGCG on D-galactosamine-induced injury in primary culture of rat hepatocytes. Hepatocytes were incubated with EGCG at concentrations of 1.25-10 μM and toxic D-galactosamine (GalN) for 24 hrs. Alternatively, hepatocytes were pretreated with EGCG for 24 hrs, and then incubated with EGCG and GalN for further 24 hrs. Cytotoxicity was analysed by lactate dehydrogenase activity, functional capacity by albumin production. Oxidative stress was evaluated from a production of malondialdehyde and glutathione content in the cells. EGCG protected hepatocytes against GalN-induced cytotoxicity but preventive treatment of intact hepatocytes with EGCG was required to diminish the development of hepatocyte injury. Oxidative stress induced in our study seems to overcome the ability of hepatocytes to improve GSH depletion and albumin production. Prolongation of the pretreatment with EGCG could be a promising strategy leading to amelioration of its hepatoprotective effect. and Alena Moravcová, Zuzana Červinková, Otto Kučera, Vojtěch Mezera, Halka Lotková

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Relation:

Acta medica (Hradec Králové) / Universitas Carolina, Facultas Medica Hradec Králové--MED00010947

Source:

Acta medica (Hradec Králové) | 2014 Volume:57 | Number:1

Harvested from:

CDK

Metadata only:

false

Date:

2014