A novel experimental model of acute respiratory distress syndrome in pig

Title:

A novel experimental model of acute respiratory distress syndrome in pig

Creator:

Otáhal, M., Mlček, M., Vítková, I., and Kittnar, O.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bddc1e97-3259-47cb-add6-698f7047443e
uuid:bddc1e97-3259-47cb-add6-698f7047443e

Subject:

Acute respiratory distress syndrome, Animal model, Pig, and Lavage

Type:

article, model:article, and TEXT

Description:

Acute respiratory distress syndrome (ARDS) is severe medicalcondition occurring in critically ill patients and with mortality of 33-52 % is one of the leading causes of death in critically ill patients. To better understand pathophysiology of ARDS and to verify novel therapeutical approaches a reliable animal model is needed. Therefore we have developed modified lavage model of ARDS in the pig. After premedication (ketamine and midazolam)35 healthy pigs were anesthetized (propofol, midazolam, morphin, pipecuronium) and orotracheally intubated and ventilated. Primary ARDS was induced by repeated cycles of lunglavage with a detergent Triton X100 diluted in saline (0.03 %) heated to 37 °C preceded by pre -oxygenation with 100 % O2. Single cycle included two subsequent lavages followed by detergent suction. Eachcyclewas followed by hemodynamic andventilation stabilization for approx. 15 min, with eventualadministration of vasopressors according to an arterial bloodpressure. The lavage procedure was repeated until the paO2/FiO2index after stabilization remained below 100 at PEEP 5 cm H2O. In 33 pigs we have achieved the desired degree of severe ARDS(PaO2/FiO2<100). Typical number of lavages was 2-3 (min. 1,max.5). Hemodynamictolerance and the need for vasopressors were strongly individual. In remainingtwo animalsan unmanageable hypotension developed. For other subjects theexperimental ARDS stability was good and allowed reliablemeasurement for more than 10 h. The present model of theARDS is clinically relevant and thus it is suitable for further research of the pathophysiology and management of this serious medical condition.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2016 Volume:65 | Number:Suppl 5

Harvested from:

CDK

Metadata only:

false