CT imaging and spontaneous behavior analysis after osmotic blood-brain barrier opening in wistar rat

Title:

CT imaging and spontaneous behavior analysis after osmotic blood-brain barrier opening in wistar rat

Creator:

Petr Kozler, Vladimír Riljak, Kateřina Jandová, and Pokorný, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bc7252b2-9e13-481a-b408-921ddf7c44ff
uuid:bc7252b2-9e13-481a-b408-921ddf7c44ff
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, hematoencefalická bariéra, počítačová tomografie, blood-brain barrier, computed tomography, brain edema, osmotic BBB disruption, CT, open field test, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

In our previous experiments we demonstrated that osmotic opening of the blood brain barrier (BBB) in rats by administration of mannitol into the internal carotid artery leads to cerebral edema. The aim of this study was to confirm objectively the development of brain edema and determine whether it affects spontaneous locomotor activity in rats (SLA). Brain edema was verified by computer tomography (CT) examination of the brain and SLA was observed during open field test. Twenty four adult male rats were divided into four groups of six: (1) control animals (C), (2) controls with anesthesia (CA), (3) controls with sham surgery (CS), (4) experimental - osmotic opening of the BBB (MA). Osmotic BBB disruption manifested by reducing the density of brain tissue (hypodensity), suggesting a higher water content in the brain tissue. SLA was compared between C, CA, CS and MA groups and between MA and CA groups. Significant difference was found only between the control group and MA group. In the first 30 min of the examination, rats after the mannitol administration revealed a marked limitation of spontaneous locomotor activity. Experimental results demonstrated reduction of spontaneous locomotor activity in rats with induced brain edema., P. Kozler, V. Riljak, K. Jandová, J. Pokorný., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2014 Volume:63 | Number:Suppl 4

Harvested from:

CDK

Metadata only:

false