Saturating irradiance-induced photoinhibition without monomerisation of photosystem 2 dimer in soybean leaves

Title:

Saturating irradiance-induced photoinhibition without monomerisation of photosystem 2 dimer in soybean leaves

Creator:

Cai, Shi-Qing, Chen, Gen-Yun, Zhang, Hai-Bo, and Xu, Da-Quan

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bc6cd0eb-ddf2-4f1b-b007-b49e490a73c4
uuid:bc6cd0eb-ddf2-4f1b-b007-b49e490a73c4
issn:0300-3604
doi:10.1023/A:1021341623339

Subject:

dimer, Glycine max, light harvesting complex 2, monomerisation, photosystem 2, and proteins

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The oligomeric state of photosystem 2 (PS2) complex in soybean leaves treated with saturating irradiance was studied by non-denaturing polyacrylamide gel electrophoresis (PAGE) and gel filtration chromatography. PS2 dimers resolved by non-denaturing PAGE accounted for about 75 % of total PS2 complex and there was no significant difference in the ratio of PS2 dimer to monomer between samples from saturating irradiance-treated and fully dark-adapted leaves. Furthermore, BBY particles were resolved into four chlorophyll-enriched fractions by gel filtration chromatography. From their molecular masses and protein components, these fractions were deduced to be PS2 dimer, PS2 monomer, oligomeric light-harvesting complex 2 (LHC2), and monomeric LHC2. Also, no change in the proportion of PS2 dimer in total PS2 was observed in the granal region of thylakoid membranes from soybean leaves after saturating irradiation. Hence the dimer is the predominant natural form of PS2 in vivo and no monomerisation of PS2 dimer occurs during saturating irradiance-induced photoinhibition in soybean leaves. and Shi-Qing Cai ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

215-220

Source:

Photosynthetica | 2002 Volume:40 | Number:2

Harvested from:

CDK

Metadata only:

false