Development of a PCR for Borrelia burgdorferi sensu lato, targeted on the groEL gene

Title:

Development of a PCR for Borrelia burgdorferi sensu lato, targeted on the groEL gene

Creator:

Chiappa, Giulia, Cafiso, Alessandra, Monza, Elisa, Serra, Valentina, Olivieri, Emanuela, Romeo, Claudia, and Bazzocchi, Chiara

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b43f00e4-780e-4f57-8aef-48701527b9e1
uuid:b43f00e4-780e-4f57-8aef-48701527b9e1
doi:10.14411/fp.2020.026

Subject:

Lyme diseases, Ixodes ricinus, qualitative PCR, detection, and Real-time PCR

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Borrelia burgdorferi sensu lato (s.l.) is the etiological agent of Lyme disease, transmitted by ticks of the genus Ixodes Latreille. Diagnosis of Lyme disease in humans is often difficult and a detailed knowledge of the circulation of B. burgdorferi s.l. in tick hosts is therefore fundamental to support clinical procedures. Here we developed a molecular approach for the detection of B. burgdorferi s.l. in North Italian Ixodes ricinus (Linnaeus). The method is based on the amplification of a fragment of the groEL gene, which encodes a heat-shock protein highly conserved among B. burgdorferi s.l. species. The tool was applied in both qualitative and Real-time PCR approaches testing ticks collected in a North Italian area. The obtained results suggest that this new molecular tool could represent a sensitive and specific method for epidemiological studies aimed at defining the distribution of B. burgdorferi s.l. in I. ricinus and, consequently, the exposure risk for humans.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

1-5

Source:

Folia parasitologica | 2020 Volume:67 | Number:1

Harvested from:

CDK

Metadata only:

false