Dehydroepiandrosterone reduced lipid droplet accumulation via inhibiting cell proliferation and improving mitochondrial function in primary chicken hepatocytes

Title:

Dehydroepiandrosterone reduced lipid droplet accumulation via inhibiting cell proliferation and improving mitochondrial function in primary chicken hepatocytes

Creator:

Li, Long-Long, Wang, Dian, Ge, Chong-Yang, Yu, Lei, Zhao, Jin-Long, and Ma, Hai-Tian

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b073482c-7b90-46cf-9102-ca9a65e82acb
uuid:b073482c-7b90-46cf-9102-ca9a65e82acb
issn:0862-8408

Subject:

metabolismus lipidů, růst buněk, lipid metabolism, cell growth, dehydroepiandrosterone, membrane permeability, mitochondrial enzyme, 14, and 612

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

Dehydroepiandrosterone (DHEA) possesses fat-reducing effect, while little information is available on whether DHEA regulates cell proliferation and mitochondrial function, which would, in turn, affect lipid droplet accumulation in the broiler. In the present study, the lipid droplet accumulation, cell proliferation, cell cycle and mitochondrial membrane potential were analysis in primary chicken hepatocytes after DHEA treated. The results showed that total area and counts of lipid droplets were significantly decreased in hepatocytes treated with DHEA. The cell viability was significantly increased, while cell proliferation was significantly inhibited in a dose-dependent manner in primary chicken hepatocytes after DHEA treated. DHEA treatment significantly increased the cell population in S phase and decreased the population in G2/M in primary chicken hepatocytes. Meanwhile, the cyclin A and cyclin-dependent kinases 2 (CDK2) mRNA abundance were significantly decreased in hepatocytes after DHEA treated. No significant differences were observed in the number of mitochondria, while the mitochondrial membrane permeability and succinate dehydrogenase (SDH) activity were significantly increased in hepatocytes after DHEA treated. In conclusion, our results demonstrated that DHEA reduced lipid droplet accumulation by inhibiting hepatocytes proliferation and enhancing mitochondrial function in primary chicken hepatocytes., Long-Long Li, Dian Wang, Chong-Yang Ge, Lei Yu, Jin-Long Zhao, Hai-Tian Ma., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2018 Volume:67 | Number:3

Harvested from:

CDK

Metadata only:

false