Inhibitory effect of t-butyl hydroperoxide on mitochondrial oxidative phosphorylation in isolated rat hepatocytes

Title:

Inhibitory effect of t-butyl hydroperoxide on mitochondrial oxidative phosphorylation in isolated rat hepatocytes

Creator:

Pavla Křiváková, Anna Lábajová, Zuzana Červinková, and Zdeněk Drahota

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:ad6d5f02-8011-47b9-a4f9-9af39da912d5
uuid:ad6d5f02-8011-47b9-a4f9-9af39da912d5
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, patologická fyziologie, jaterní buňky, pathological physiology, liver cells, oxidační fosforylace, oxidative phosphorylation, t-butyl hydroperoxide, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Using high-resolution oxygraphy, we tested the changes of various parameters characterizing the mitochondrial energy provision system that were induced by peroxidative damage. In the presence of succinate as respiratory substrate, 3 mM t-butyl hydroperoxide increased respiration in the absence of ADP, which indicated partial uncoupling of oxidative phosphorylation. Low activity of coupled respiration was still maintained as indicated by the ADP-activated and oligomycin-inhibited respiration. However, during the incubation the phosphorylative capacity decreased as indicated by the continuous decrease of the mitochondrial membrane potential. Under these experimental conditions the maximum capacity of the succinate oxidase system was inhibited by 50 % in comparison with values obtained in the absence of t-butyl hydroperoxide. Our data thus indicate that the oxygraphic evaluation of mitochondrial function represents a useful tool for evaluation of changes participating in peroxidative damage of cell energy metabolism., P. Křiváková, A. Lábajová, Z. Červinková, Z. Drahota., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:1

Harvested from:

CDK

Metadata only:

false