Interleukin-10 is differentially expressed in the small intestine and the colon experiencing chronic inflammation and ulcerative colitis induced by dextran sodium sulfate in young pigs

Title:

Interleukin-10 is differentially expressed in the small intestine and the colon experiencing chronic inflammation and ulcerative colitis induced by dextran sodium sulfate in young pigs

Creator:

Lackeyram, D., Young, D., Kim, C. J., Yang, C., Archbold, T. L., Mine, Y., and Fan, M. Z.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:ad471c0f-06ee-4d4a-8e09-253f73ba97d3
uuid:ad471c0f-06ee-4d4a-8e09-253f73ba97d3
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, záněty, cytokiny, inflammations, cytokines, chronic bowel inflammation, gut permeability, IL-10, colonic ulcerative colitis, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

ntestinal inflammation induced with dextran sodium sulfate (DSS) is used to study acute or chronic ulcerative colitis in animal models. Decreased gut tissue anti-inflammatory cytokine IL-10 concentration and mRNA abundance are associated with the development of chronic bowel inflammation. Twelve piglets of 3 days old were fitted with an intragastric catheter and randomly allocated into control and DSS groups by administrating either sterile saline or 1.25 g of DSS/ kg body weight (BW) in saline per day, respectively, for 10 days. Growth rate and food conversion efficiency were reduced (p<0.05) in the DSS piglets compared with the control group. Quantitative histopathological grading of inflammation in the jejunum and colon collectively showed that the DSS tr eatment resulted in 12 fold greater (p<0.05) inflammation severity scoring in the colon than in the jejunum, indicative of chronic ulcerative colitis in the colon. Upper gut permeability endpoint was 27.4 fold high er (p<0.05) in the DSS group compared with the control group. The DSS group had higher concentrations and mRNA abundances (p<0.05) of TNF - α and IL-6 in the jejunal and colonic tissues compared with the control group. Colonic concentration and mRNA abundanc e of IL-10 were reduced (p<0.05), however, jejunal IL-10 mRNA abundance was increased (p<0.05) in the DSS group compared with the control group. In conclusion , administration of DSS at 1.25 g/kg BW for 10 days respectively induced acute inflammation in th e jejunum and chronic inflammation and ulcerative colitis in the colon with substantially decreased colonic concentration and mRNA abundance of IL-10 in the young pigs, mimicking the IL-10 expression pattern in humans associated with chronic bowel inflamma tion., D. Lackeyram, D. Young, C. J. Kim, C. Yang, T. L. Archbold, Y. Mine, M. Z. Fan., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2017 Volume:66 | Number:1

Harvested from:

CDK

Metadata only:

false