Genotypic, phenotypic, biochemical, physiological and pathogenicity-based categorisation of Acanthamoeba strains

Title:

Genotypic, phenotypic, biochemical, physiological and pathogenicity-based categorisation of Acanthamoeba strains

Creator:

Khan, Naveed Ahmed and Tareen, Noor Khan

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9fb96277-44b3-4597-bc8a-25f1142e592c
uuid:9fb96277-44b3-4597-bc8a-25f1142e592c
doi:10.14411/fp.2003.017

Subject:

Acanthamoeba, cytotoxicity, differentiation, keratitis, corneal epithelial cells polyphaga, CCAP 1501/3c and ATCC 30871, using morphological, biochemical, physiological, and molecular and cytotoxicity

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The genus Acanthamoeba includes more than 20 morphological species, but classification is problematical. Recently, the discovery of substantial interstrain differences in ribosomal DNA (rDNA) sequences has prompted questions about the relatedness of strains of the same species. In this study, therefore, we have investigated relationships between two isolates of A. polyphaga, CCAP 1501/3c and ATCC 30871, using morphological, biochemical, physiological, molecular and cytotoxicity assays. We observed that A. polyphaga ATCC 30871 exhibited up to six arms in endocyst while A. polyphaga CCAP 1501/3c exhibited a maximum of 5 arms thus indicating their position in group 2 and 3, respectively. Acanthamoeba polyphaga ATCC 30871 exhibited growth at 37°C and growth on 1M mannitol plates while A. polyphaga CCAP 1501/3c did not. In addition, both isolates exhibited differences in isoenzyme banding patterns and rDNA restriction fragment polymorphisms. More importantly, A. polyphaga ATCC 30871 produced cytotoxicity on corneal epithelial cells while A. polyphaga CCAP 1501/3c had no effects, suggesting differences in pathogenicity. Thus, all the results provide evidence for significant differences between the strains and further provided the basis for reclassification of the isolates. Implications of these results in the clinical diagnosis of pathogenic Acanthamoeba are discussed.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

97-104

Source:

Folia parasitologica | 2003 Volume:50 | Number:2

Harvested from:

CDK

Metadata only:

false