Oxidative stress in the brain tissue of laboratory mice with acute post insulin hypoglycemia

Title:

Oxidative stress in the brain tissue of laboratory mice with acute post insulin hypoglycemia

Creator:

Jitka Patočková, Petr Marhol, Eva Tůmová, Miloslav Kršiak, Richard Rokyta, Stanislav Štípek, Jiřina Crkovská, and Michal Anděl

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9ea0906e-7905-4741-a36d-58e64883dd3f
uuid:9ea0906e-7905-4741-a36d-58e64883dd3f
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, hypoglykémie, hypoglycemia, malondialdehyde, Cu, Zn-superoxide dismutase, selenium-dependent glutathione peroxidase, insulin-induced hypoglycemia, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Malondialdehyde (MDA), Cu,Zn-superoxide dismutase (Cu,Zn-SOD) and selenium-dependent glutathione peroxidase (GSPHx) are currently considered to be basic markers of oxidative stress. MDA is one of the end-products of the peroxidation of membrane lipids, whereas enzymes Cu,Zn-SOD and GSHPx belong to the natural antioxidants. The role of oxygen free radicals in the pathogenesis of many diseases is well documented. The aim of this study was to ascertain the influence of insulin-induced acute hypoglycemia on oxidative stress in the brain tissue. Hypoglycemia was induced in ICR mice by intraperitoneal administration of insulin at a dose 24 IU/kg. There was a correlation between the severity of hypoglycemia and the levels of MDA, Cu,Zn-SOD and GSHPx. The results showed that in severe hypoglycemia (serum glucose concentration below 1.0 mmol/l) the lipoperoxidation in brain tissue expressed as the level of MDA was higher in comparison with normoglycemic controls (glycemia around 3.7 mmol/l) as well as in comparison with the levels of MDA during moderate hypoglycemia (glycemia ranging between 1-2 mmol/l). This indicates the enhancement of lipoperoxidation in the brain tissue during severe hypoglycemia. However, both enzymes - Cu,Zn-SOD or GSHPx - did not show a similar tendency., J. Patočková, P. Marhol, E. Tůmová, M. Kršiak, R. Rokyta, S. Štípek, J. Crkovská, M. Anděl., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2003 Volume:52 | Number:1

Harvested from:

CDK

Metadata only:

false