Characterization and functional analysis of the serpin-10 gene from oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae)

Title:

Characterization and functional analysis of the serpin-10 gene from oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae)

Creator:

Kausar, Saima, Qian, Cen, Abbas, Muhammad Nadeem, Zhu, Bao-Jian, Liu, Ya, Wang, Lei, Wei, Guo-Qing, Sun, Yu, and Liu, Chao-Liang

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9be69f9f-24a3-4a5e-8b32-38fcd47d3e16
uuid:9be69f9f-24a3-4a5e-8b32-38fcd47d3e16
issn:1210-5759
doi:10.14411/eje.2017.055

Subject:

Zoologie, motýli, butterflies, Lepidoptera, Saturniidae, Antheraea pernyi, serpin, innate immunity, haemolymph protein, 2, and 59

Type:

article, články, journal articles, model:article, and TEXT

Format:

print, počítač, and online zdroj

Description:

Serpin is a broadly distributed superfamily of proteins that have a crucial role in regulating various immune reactions. Herein we identified a serpin-10 gene from Antheraea pernyi that encodes a 1557 amino acid residue protein with a predicted molecular weight of 58.76 kDa. Recombinant Apserpin-10 protein was expressed in a prokaryotic expression system (Escherichia coli) and the purified protein was used to prepare rabbit anti-Apserpin-10 polyclonal antibodies. Quantitative real-time polymerase chain reaction and western blot analysis indicate that Apserpin-10 was transcribed in all the tissues examined, including haemolymph, malpighian tubules, fat body, silk gland, integument and mid gut; the greatest expression level of Apserpin-10 was recorded in the fat body and haemocytes. The comparison of different developmental stages showed that Apserpin-10 transcript level was highest in 5th instar larvae, while the lowest expression was recorded at the egg stage. We also investigated the expression patterns of Apserpin-10 in fat body and haemocyte samples, following administration of heat-inactivated gram-positive bacteria (Micrococcus luteus), gram negative bacteria (Escherichia coli), a fungus (Beauveria bassiana) and virus (nuclear polyhedrosis virus, NPV). A substantial up-regulation of Apserpin-10 expression was recorded following pathogen challenge in both the tissues tested. Further the knock down of Apserpin-10 led to down regulation of antimicrobial peptide genes. Altogether, our results indicate that Apserpin-10 is involved in the innate immunity of A. pernyi., Saima Kausar, Cen Qian, Muhammad Nadeem Abbas, Bao-Jian Zhu, Ya Liu, Lei Wang, Guo-Qing Wei, Yu Sun, Chao-Liang Liu., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

European Journal of Entomology | 2017 Volume:114

Harvested from:

CDK

Metadata only:

false