Influence of low temperatures on photosystem II photochemistry and expression of the NADPH:protochlorophyllide oxidoreductase in the alpine, subnival perennial, Chorispora bungeana

Title:

Influence of low temperatures on photosystem II photochemistry and expression of the NADPH:protochlorophyllide oxidoreductase in the alpine, subnival perennial, Chorispora bungeana

Creator:

Li, Y. H., Sun, Z. L., Xu, X. L., Jin, M., Liu, Y. J., Zhang, H., and An, L. Z.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9ade862a-2a89-4add-8aa9-dcd81b771e2b
uuid:9ade862a-2a89-4add-8aa9-dcd81b771e2b
issn:0300-3604
doi:10.1007/s11099-010-0060-1

Subject:

botanika, botany, chlorophyll biosynthesis, chlorophyll fluorescence, Chorispora bungeana, low temperature, NADPH, and protochlorophyllide oxidoreductase

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Low temperature significantly influences chloroplast development and chlorophyll (Chl) biosynthesis, so effect of coldness on Chl content and Chl fluorescence characteristics was investigated in C. bungeana (Chorispora bungeana Fisch. & C.A. Mey). The levels of transcript and protein of an enzymatic step during Chl biosynthesis in response to chilling (4°C) and freezing (-4°C) were also examined in this work. Significant reduction in total Chl content was observed, but the reduction was much less at 4°C than that at -4°C. Moreover, the maximal quantum efficiency of photosystem II (PSII) photochemistry, indicated by Fv/Fm, decreased in the first 12 h, but then started to increase and reached higher levels than the control at 24 h and 48 h at 4°C, but decreased continuously at -4°C. Whereas quantum yield of PSII (ΦPSII) showed no significant difference between the chilling-stressed and the control seedlings, at -4°C, ΦPSII was markedly reduced with the prolonged treatment. In general, there were no significant responses of photochemical quenching (qP) and non-photochemical quenching (NPQ) to cold treatment. Meanwhile, the full-length cDNA of NADPH:protochlorophyllide oxidoreductase (POR, EC 1.3.1.33) was isolated and termed CbPORB (GenBank Accession No. FJ390503). Its transcript and protein content only slightly declined at 4°C, but dramatically reduced at -4°C with the time. These results strongly suggest that CbPORB possesses certain resistant characteristics and is a major player in Chl biosynthesis process involved in plant growth and development of C. bungeana under cold environmental conditions. and Y. H. Li ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

457-468

Source:

Photosynthetica | 2010 Volume:48 | Number:3

Harvested from:

CDK

Metadata only:

false