Effects of ectopically expressed hyperthermophilic archaeon (Pyrococcus furiosus) ribulose-1,5-bisphosphate carboxylase/oxygenase on tobacco photosynthesis

Title:

Effects of ectopically expressed hyperthermophilic archaeon (Pyrococcus furiosus) ribulose-1,5-bisphosphate carboxylase/oxygenase on tobacco photosynthesis

Creator:

Li, X.-G., Yang, J., Wang, R., Tang, X.-F., Meng, J.-J., Qin, H.-J., Liu, X., Guo, F., and Wan, S.-B.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:95f6c87d-9829-4684-8312-0b8614a44bd8
uuid:95f6c87d-9829-4684-8312-0b8614a44bd8
issn:0300-3604
doi:10.1007/s11099-013-0035-0

Subject:

fotosyntéza, photosynthesis, high-temperature stress, Nicotiana benthamiana, Rubisco, virus mediated ectopic expression, 2, and 581

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Pyrococcus furiosus is a hyperthermophilic archaeon. Its ribulose-1,5-bisphosphate carboxylase/oxygenase (PfRubisco) has only large subunit (L). PfRubisco has a novel (L2)5, decameric structure and it possesses higher carboxylase activity and thermotolerance. To assess the potential functionality of PfRubisco in higher plants under high-temperature stress, PfRubisco coding sequence was transiently expressed in Nicotiana benthamiana by Pea early browning virus mediated ectopic expression. The transgenic PfRubisco plants produced chlorotic yellow stripes in their leaves. Relative to the control leaves, those with yellow stripes exhibited decreased net photosynthetic rate and chlorophyll content, altered chloroplast ultrastructure, and more severe photoinhibition of both photosystem I and II. We concluded that the ectopic expression of PfRubisco might disrupt the chloroplast development and function in N. benthamiana. The potential cause of the disruption was discussed. and X. -G. Li ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

387-394

Source:

Photosynthetica | 2013 Volume:51 | Number:3

Harvested from:

CDK

Metadata only:

false