The influence of interleukin-1β on γ-glutamyl transpepidase activity in rat hippocampus

Title:

The influence of interleukin-1β on γ-glutamyl transpepidase activity in rat hippocampus

Creator:

Kiaser, M., Mareš, V., Šťastný , F., Bubeníková-Valešová, V., Lisá, V., Suchomel, P., and Balcar, V.J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:94d7e294-7dfb-42a6-bbf3-77cdf1c7bce0
uuid:94d7e294-7dfb-42a6-bbf3-77cdf1c7bce0

Subject:

Pro-inflammatory cytokine, Rat hippocampus, γ-glutamyl transferase, Blood-brain barrier, Reactive oxygen species, and Schizophrenia

Type:

article, model:article, and TEXT

Description:

Brain infections as well as peripheral challenges to the immune system lead to an increased production of interleukin-1beta (IL-1b), a cytokine involved in leukocyte-mediated breakdown of the blood-brain barrier. The effects of IL-1b have been reported to depend on whether the route of administration is systemic or intracerebral. Using 50-day-old male rats, we compared the effects of IL-1b on brain γ-glutamyl transpeptidase (GGT; an enzymatic marker of brain capillary endothelium) at 2, 24 and 96 h after either an intravenous (i.v.) injection of 5 μg IL-1β or an intracerebroventricular (i.c.v. - lateral ventricle) infusion of 50 ng IL-1β. When the i.v. route was used, the GGT activity underwent small but significant changes; decreasing in the hippocampus 2 h after the i.v. injection, increasing 24 h later and returning to control levels at 96 h. No significant changes in the hippocampal GGT activity were observed at 2 and 24 h following the i.c.v. infusion. The GGT activity in the hypothalamus remained unchanged regardless of the route of IL-1b administrations. Similar changes in GGT activity were revealed histochemically. The labeling was found mainly in the capillary bed, the changes being most evident in the hippocampal stratum radiatum and stratum lacunosum-moleculare. A transient increase in GGT activity at 24 h, together with a less sharp delineation of GGT-stained vessels, may reflect IL-1b induced increased turnover of glutathione and/or oxidative stress, that may in turn, be related to altered permeability of the blood-brain barrier in some neurological and mental disorders, including schizophrenia.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2006 Volume:55 | Number:4

Harvested from:

CDK

Metadata only:

false