Proteolytic enzymes secreted by larval stage of the parasitic nematode Trichinella spiralis

Title:

Proteolytic enzymes secreted by larval stage of the parasitic nematode Trichinella spiralis

Creator:

Todorova, Valentina K.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:8d56adfb-b717-461a-a8a8-99ad65937fcf
uuid:8d56adfb-b717-461a-a8a8-99ad65937fcf
urnnbn:urn:nbn:cz:aba007-000aul

Subject:

antibody inhibition, Trichinella spiralis, serine proteinases, larval stage, and excreted/secreted products

Type:

model:internalpart and TEXT

Format:

print

Description:

Excretory/secretory products (ES), collected from in vitro cultures of muscle larvae (l-l) of Trichinella spiralis (Owen, 1835) were examined for the presence of proteolytic enzymes. Several discrete proteinases in the size range of 25-55 kDa were identified by substrate gel electrophoresis and were characterised according to pH optima, substrate specificity and inhibitor sensitivity using azocasein assay. Serine, cystcinc and metalloproleinases active at pi I 5-7 were identified. The serine proteinases were found to predominate and some of them were found to be specific for the larval stage of the parasite. The results from the substrate analysis indicated the presence of collagenolytic and elastolytic activities. The proteinase activity was inhibited by IgG isolated from T. s/nrato-infcctcd mice, an observation of relevance to understanding host/parasite interactions and, ultimately, the development of anti-Trichinella vaccine.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

141-145 and 63-67

Source:

Folia parasitologica | 2000 Volume:47 | Number:2

Harvested from:

CDK

Metadata only:

false