A preliminary evaluation of drug biotransformation in hepatocytes of genetically defined rat strains

Title:

A preliminary evaluation of drug biotransformation in hepatocytes of genetically defined rat strains

Creator:

Hynie, S., Křen, V., Bílá, V., Mráz, M., Gaier, N., Kameníková, L., and Farghali, H.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:8d1c3538-2b98-4a7e-b8e7-a7ba671bccf1
uuid:8d1c3538-2b98-4a7e-b8e7-a7ba671bccf1

Subject:

hepatocytes, spontaneously hypertensive rat, isoprenaline-sensitive rat, and isoprenaline-resistant rat

Type:

article, model:article, and TEXT

Description:

This study was directed to use the genetically developed isoprenaline-sensitive (S), isoprenaline-resistant (R) and spontaneous hypertensive rats (SHR) as standard diseased animal models for in vitro liver function evaluation of drug biotransformation. Hepatic hexobarbital hydroxylase and glutathione transferase (GST) were evaluated by using hexobarbital and l-chloro-2,4-dinitrobenzene (CDNB) as substrates, at concentrations of 0.21 mmol/l and 1 mmol/l, respectively. The assay was conducted by using isolated hepatocytes in suspension and hepatocytes in a bioreactor configuration. The data demonstrate that there are certain cellular pharmacokinetic differénces in hexobarbital hydroxylase and GST activities in hepatocytes obtained from Wistar, SHR, R and S strains which can be better demonstrated, when using the model of perfused and immobilized hepatocytes.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1994 Volume:43 | Number:2

Harvested from:

CDK

Metadata only:

false