L-type and T-type Ca2+ Current in Cultured Ventricular Guinea Pig Myocytes

Title:

L-type and T-type Ca2+ Current in Cultured Ventricular Guinea Pig Myocytes

Creator:

Zorn-Pauly, K., Schaffer, P., Pelzmann, B., Bernhart, E., Lang, P., and Koidl, B.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:882fe6b0-665b-4049-926d-2d54b0efd5db
uuid:882fe6b0-665b-4049-926d-2d54b0efd5db

Subject:

Ventricular myocytes, Short-term culture, L-type Ca2+ channel, and T-type Ca2+ channel

Type:

article, model:article, and TEXT

Description:

The aim of this investigation was to study L-type and T-type Ca2+ current (ICaL and ICaT) in short-term cultured adult guinea pig ventricular myocytes. The isolated myocytes were suspended in serum-supplemented medium up to 5 days. Using whole-cell patch clamp techniques ICaL and ICaT were studied by applying voltage protocols from different holding potentials (–40 and –90 mV). After 5 days in culture the myocytes still showed their typical rod shaped morphology but a decline in cell membrane capacitance (26 %). The peak density of ICaT was reduced significantly between day 0 (–1.60.37 pA/pF, n=9) and day 5 (–0.40.13 pA/pF, n=11), whereas peak ICaL density revealed no significant differences during culturing. The ICaT/ICaL ratio dropped from 0.13 at day 0 to 0.05 at day 5. Compared with day 0 ICaL the steady state inactivation curve of day 1, day 3 and day 5 myocytes was slightly shifted to more negative potentials. Our data indicate that guinea pig ventricular L-type and T-type Ca2+ channels are differently regulated in culture.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2004 Volume:53 | Number:4

Harvested from:

CDK

Metadata only:

false