Several functions of immune cells changed in mice with oxidative stress caused by endotoxin

Title:

Several functions of immune cells changed in mice with oxidative stress caused by endotoxin

Creator:

Víctor, V. M. and La Fuente, M. de

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:81263844-cd56-456a-abcb-0a09fe8268dc
uuid:81263844-cd56-456a-abcb-0a09fe8268dc
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, cytotoxic activity, leukocytes, proinflammatory mediators, proliferation, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

We have studied natural killer (NK) activity, lymphoproliferative response, the release of several cytokines (IL-2, TNFa and IL-1b) and the ROS production in peritoneal leukocytes obtained 0, 2, 4, 12 and 24 h after lipopolysaccharide (LPS) injection. Lethal septic shock (100 % mortality occurred at 30 h after LPS administration) was caused in female BALB/c mice by intraperitoneal injection of 100 mg/kg of E. coli LPS. Cytotoxicity and lymphoproliferation assay were preformed together with the measurement of IL-1b, IL-2 and TNFa production, and quantification of ROS. Natural killer activity, spontaneous lymphoproliferative response, IL-2, TNFa, IL-b release and ROS production were increased after LPS injection. In conclusions, ROS and proinflammatory mediators produced by immune cells in response to LPS are involved in the oxidative stress of endotoxic shock. This oxidative state alters some functional characteristics of leukocytes (proliferation and NK activity)., V. M. Víctor, M. De la Fuente., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2003 Volume:52 | Number:6

Harvested from:

CDK

Metadata only:

false