Spermatozoon and spermiogenesis in Mesocoelium monas (Platyhelminthes: Digenea): ultrastructure and epifluorescence microscopy of labelling of tubulin and nucleus

Title:

Spermatozoon and spermiogenesis in Mesocoelium monas (Platyhelminthes: Digenea): ultrastructure and epifluorescence microscopy of labelling of tubulin and nucleus

Creator:

Iomini, Carlo, Mollaret, Isabelle, Albaret, Jean-Louis, and Jistine, Jean-Lou

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:8046ad4c-e96b-46b9-b484-396e0cea3e92
uuid:8046ad4c-e96b-46b9-b484-396e0cea3e92
urnnbn:urn:nbn:cz:aba007-000dmq

Subject:

ultrastructure, immunocytochemistry, Platyhelminthes, Digenea, spermatozoon, and tubulin

Type:

model:internalpart and TEXT

Format:

print

Description:

Spermiogenesis and the spermatozoon were studied in the digenean Mesocoelium monas Rudolphi, 1819 (from the toad Bufo sp. in Gabon). An ultrastructural study revealed that spermiogenesis follows the usual pattern found in digeneans, i.e. proximo-distal fusion of axonemes with a median cytoplasmic process followed by elongation. The spermatozoon has two fully incorporated axonemes with the 9 +“1” trepaxonematan pattem. Indirect immunofluorescence localization of tubulin and fluorescent labelling of the nucleus were used to obtain additional information on the structure of the spermatozoon. It was thus shown that one of the axonemes is slightly shorter than the other (190 versus 220 pm) and that the filiform nucleus (65 pm in length) is located at the distal extremity of the spermatozoon (220 pm in length). Various monoclonal and polyclonal antibodies, specific to alpha, beta, acetylated-alpha, or general tubulin, were used and produced similar labelling.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

26-32 and 28-34

Source:

Folia parasitologica | 1997 Volume:44 | Number:1

Harvested from:

CDK

Metadata only:

false