Lectinhistochemical detection of terminal carbohydrate residues in the enteric myxozoan Enteromyxum leei parasitizing gilthead seabream Sparus aurata (Pisces: Teleostei): a study using light and transmission electron microscopy

Title:

Lectinhistochemical detection of terminal carbohydrate residues in the enteric myxozoan Enteromyxum leei parasitizing gilthead seabream Sparus aurata (Pisces: Teleostei): a study using light and transmission electron microscopy

Creator:

Redondo, María J. and Alvarez-Pellitero, Pilar

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7fc87cc7-5044-4b4a-b89f-feef21b7f710
uuid:7fc87cc7-5044-4b4a-b89f-feef21b7f710
doi:10.14411/fp.2009.030

Subject:

Myxosporea, lectins, lectin-gold electron microscopy, and histochemistry

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The presence of terminal carbohydrate residues in Enteromyxum leei (Diamant, Lom et Dyková, 1994) Palenzuela, Redondo et Álvarez-Pellitero, 2002 stages in gilthead seabream intestines was studied at light microscopy (LM) and transmission electron microscopy (TEM) level using lectin histochemical techniques. Abundant mannose and/or glucose residues were demonstrated by the intense staining caused by binding of biotinylated concanavalin A (Con A), at both LM and TEM. A clear positivity was also obtained with Ulex europaeus (UEA I) agglutinin specific for fucose residues. Both lectins stained E. leei proliferative and sporogonic stages, though glycan patterns varied between these developmental stages. Wheat germ agglutinin (WGA) and Bandeiraea simplicifolia lectin I (BSL I) recognised only structures in the sporogonic stages. Faint labelling occurred with Glycine max (SBA) lectin. No staining was obtained with Sambucus nigra (SNA) agglutinin. The TEM studies demonstrated a restricted presence of N‑acetyl-D-galactosamine and α-D-galactose, whereas glucose/mannose and fucose, the dominant structures, were also present at the parasite membranes and host-parasite interface, suggesting a role in host-parasite interaction.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

259-267

Source:

Folia parasitologica | 2009 Volume:56 | Number:4

Harvested from:

CDK

Metadata only:

false