Fiber type composition of unoperated rat soleus and extensor digitorum longus muscles after unilateral isotransplantation of a foreign muscle in long-term experiments

Title:

Fiber type composition of unoperated rat soleus and extensor digitorum longus muscles after unilateral isotransplantation of a foreign muscle in long-term experiments

Creator:

Tomáš Soukup, Smerdu, V., and Gisela Zachařová

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7ee7eab9-f127-41f2-8d57-52e0343b5d7b
uuid:7ee7eab9-f127-41f2-8d57-52e0343b5d7b

Subject:

Fyziologie člověka a srovnávací fyziologie, imunohistochemie, svaly, transplantace, chirurgické operace, immunohistochemistry, muscles, transplantation, surgical procedures, muscle fiber types, mATPase and immunohistochemistry, myosin heavy chains, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

We examined the effects of the unilateral heterochronous isotransplantation on the fiber type composition and myosin heavy chain (MyHC) isoform content of unoperated slow soleus and fast extensor digitorum longus muscles of female inbred Lewis strain rats. Comparison was made between “control” unoperated muscles of experimental rats (after intramuscular transplantation surgery) with the corresponding muscles of completely naive (unoperated) rats of three age groups (5-, 8- and 14-month-old). This was done in order to ascertain whether these muscles can be used as reliable controls to the transplanted and host muscles for our ongoing grafting experiments. The fiber type composition was determined by assessing the histochemical reaction for myofibrillar adenosine triphosphatase, the MyHC isoform content was determined immunocytochemically using monoclonal antibodies specific to different MyHC isoforms and by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Our experiments show that the heterochronous intramuscular isotransplantation procedure had no significant effect on the fiber type composition and MyHC isoform content of the “control” unoperated muscles of the experimental rats when compared to the corresponding muscles of the naive animals. Furthermore, the duration and type of isotransplantation did not also lead to differences among corresponding “control” muscles of experimental animals. We conclude that the unoperated muscles of the experimental rats can be used as controls in our current transplantation project dealing with long-term grafting experiments., T. Soukup, V. Smerdu, G. Zachařová., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2009 Volume:58 | Number:2

Harvested from:

CDK

Metadata only:

false