Band 3 protein function in teleost fish erythrocytes: effects of oxygenation-deoxygenation

Title:

Band 3 protein function in teleost fish erythrocytes: effects of oxygenation-deoxygenation

Creator:

Russo, A., Tellone, E., Ficarra, S., Giardina, B., Bellocco, E., Lagana, C., Leuzzi, U., Arnošt Kotyk, and Galtieri, A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7c6d3b33-7804-4f85-a09e-7537654dfc67
uuid:7c6d3b33-7804-4f85-a09e-7537654dfc67
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, biochemie, fyziologie, erytrocyty, biochemistry, physiology, erythrocytes, hemoglobin, band 3 protein, anion transport, oxygenation-deoxygenation cycle, 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

During vertebrate evolution, structural changes in red blood cells (RBC) and hemoglobin (Hb), have probably resulted in the importance of blood carbon dioxide transport. The chloride/bicarbonate exchange across the RBC membrane, which is an integral part of the blood CO2 transport process in vertebrates, has been examined on two different species of teleost fish, Euthynnus alletteratus and Thunnus thynnus, at several oxygenation states of erythrocyte HOS (high-oxygenation state, about 90 % of saturation) and LOS (low-oxygenation state, about 15 % of saturation). The results were compared with those observed in human RBC under the same experimental conditions and with the chicken (Gallus gallus) erythrocytes, which have particular modifications at the N-terminus of the band 3 protein (B3). In fish the kinetic measurements have shown a different anion transport in several oxygenation states of erythrocytes, indicating that also at lower levels of vertebrate evolution there exists a modulation of the anionic flow affected by oxygen. The functional correlation of anion transport to changes of parts of the hemoglobin sequence responsible for alterations in the interactions with the cytoplasmic domain of band 3 protein (cdb3) allowed us to suggest a hypothesis about fish physiology. The highest values of kinetic measurements observed in fish have been attributed to the metabolic need of the RBC in response to the removal of CO2 that in teleosts is also of endogenous origin., A. Russo, E. Tellone, S. Ficarra, B. Giardina, E. Bellocco, G. Lagana, U. Leuzzi, A. Kotyk, A. Galtieri., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2008 Volume:57 | Number:1

Harvested from:

CDK

Metadata only:

false