Altered expression of ORAI and STIM isoforms in activated human cardiac fibroblasts

Title:

Altered expression of ORAI and STIM isoforms in activated human cardiac fibroblasts

Creator:

Čendula, Róbert, Chomaničová, Nikola, Adamičková, Adriana, Gažová, Andrea, Kyselovič, Ján, and Máťuš, Marek

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7542229a-c53e-4e47-a810-7da05174b610
uuid:7542229a-c53e-4e47-a810-7da05174b610
issn:0862-8408
doi:10.33549/physiolres.934771

Subject:

fyziologie člověka, kardiologie, human physiology, cardiology, Human cardiac fibroblast (HCF), SOCE (Store-operated calcium entry), Orai, STIM, 14, and 612

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Cardiac fibrotization is a well-known process characteristic of many cardiac pathological conditions. The key element is excessive activation of cardiac fibroblasts, their transdifferentiation into myofibroblasts, increased production, and accumulation of extracellular matrix proteins, resulting in cardiac stiffness. The exact cellular mechanisms and molecular components involved in the process are not fully elucidated, but the SOCE mechanism could play an important role. Its key molecules are the molecular sensor of calcium in ER/SR - STIM and the highly selective calcium channels Orai located in the plasma membrane. This study aims to evaluate selected SOCEassociated genes in the activation of HCF cell culture by several known substances (phenylephrine, isoprenaline) that represent cardiovascular overload. After cell cultivation, cell medium was collected to measure the soluble collagen content. From the harvested cells, qRT-PCR was performed to determine the mRNA levels of the corresponding genes. The activation of cells was based on changes in the relative expression of collagen genes as well as the collagen content in the medium of the cell culture. We detected an increase in the expression of the Orai2 isoform, a change in the Orai1/Orai3 ratio and also an increase in the expression of the STIM2 isoform. These results suggest an increased activation of the SOCE mechanism under stress conditions of fibroblasts, which supports the hypothesis of fibroblast activation in pathological processes by altering calcium homeostasis through the SOCE mechanism., Róbert Čendula, Nikola Chomaničová, Adriana Adamičková, Andrea Gažová, Ján Kyselovič, Marek Máťuš., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

S21-S30

Source:

Physiological research | 2021 Volume:70 | Number:Supplement 1

Harvested from:

CDK

Metadata only:

false