Nested PCR detection of Plasmodium malariae from microscopy confirmed P. falciparum samples in endemic area of NE India

Title:

Nested PCR detection of Plasmodium malariae from microscopy confirmed P. falciparum samples in endemic area of NE India

Creator:

Dhiman, Sunil, Goswami, Diganta, Kumar, Dinesh, Rabha, Bipul, Kumar Sharma, Dhirendra, Kumar Bhola, Rakesh, Baruah, Indra, and Veer, Vijay

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7527a39c-f783-45c7-bdc7-7b9cad9f0607
uuid:7527a39c-f783-45c7-bdc7-7b9cad9f0607
doi:10.14411/fp.2013.041

Subject:

malaria, diagnostics, blood samples, OptiMAL-IT®, sensitivity, specificity, and Assam

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The present study evaluates the performance of OptiMAL-IT® test and nested PCR assay in detection of malaria parasites. A total of 76 randomly selected blood samples collected from two malaria endemic areas were tested for malaria parasites using microscopy and OptiMAL-IT® test in the field. PCR assays were performed in the laboratory using DNA extracted from blood spots of the same samples collected on the FTA™ classic cards. Of the total of 61 field confirmed malaria positive samples, only 58 (95%) were detected positive using microscopy in the laboratory. Sensitivity, specificity, positive predictive value, negative predictive value and false discovery rate of OptiMal-IT® in comparison to the microscopy were 93%, 83%, 95%, 79% and 5%, respectively. On the other hand, the sensitivity and specificity of PCR assay were 97% and 100 %, respectively, whereas positive predictive value, negative predictive value and false discovery rate were 100%, 90% and 0%, respectively. The overall performance of OptiMal-IT® and PCR assays for malaria diagnosis was 76% and 97%, respectively. PCR assay enabled the identification of infection with Plasmodium malariae Laveran, 1881 in four samples misidentified by microscopy and Plasmodium-specific antigen (PAN) identified by the OptiMAL-IT® test. In addition to the standard methods, such PCR assay could be useful to obtain the real incidence of each malaria parasite species for epidemiological perspectives.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

401-405

Source:

Folia parasitologica | 2013 Volume:60 | Number:5

Harvested from:

CDK

Metadata only:

false