Role of protoolysis and apoptosis in regression of pulmonary vascular remodeling

Title:

Role of protoolysis and apoptosis in regression of pulmonary vascular remodeling

Creator:

Riley, D. J., Thakker-Varia, S., Wilson, F. J., Poiani, G. J., and Tozzi, C. A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:746148ae-399e-410b-8038-de96a33a38b3
uuid:746148ae-399e-410b-8038-de96a33a38b3
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, apoptóza, hypertenze, apoptosis, hypertension, vascular remodeling, matrix metalloproteinase, pulmonary, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Remodeled pulmonary arteries return to normal structural conditions after the increase in pulmonary artery flow resistance is reversed. We studied whether proteolysis of extracellular matrix proteins and apoptosis occur during reversal of remodeling produced by chronic hypoxia in the rat. Main pulmonary arteries were removed at different times during a 10-day period of exposure to 10% O2 and 14 days after return to air. Content and rates of degradation of collagen and elastin as well as immunoreactive collagenase in tissue and isolated mast cells were measured. Immunoblots for collagenase and tissue inhibitor of metalloproteinases (TIMP) were performed. Apoptosis was assessed by cleavage of DNA and TUNEL assay. Excess collagen and elastin present at 10 days of hypoxia decreased to near normal levels after 3-5 days of air. Transient increases in collagenolytic and elastolytic enzyme activities accompanied the rapid decrease in matrix proteins. Mast cells containing collagenase accumulated in remodeled pulmonary arteries, and the active form of collagenase appeared at the time of peak proteolytic activity. TIMP increased during remodeling. Apoptosis was maximal 3 days after return to air. Our results suggest that activation of enzymes, which degrade matrix proteins, and apoptosis play a role in resolution of vascular remodeling., D. J. Riley, S. Thakker-Varia, F. J. Wilson, G. J. Poiani, C. A. Tozzi., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2000 Volume:49 | Number:5

Harvested from:

CDK

Metadata only:

false