Changes in the thyiakoid membrane polypeptide patterns triggered by excess Cu in rice

Title:

Changes in the thyiakoid membrane polypeptide patterns triggered by excess Cu in rice

Creator:

Lidon, F. C. and Henriques, F. S.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:6cab6199-0c30-42e7-8f6a-f2b1beafd410
uuid:6cab6199-0c30-42e7-8f6a-f2b1beafd410
urnnbn:urn:nbn:cz:aba007-000fd8

Type:

model:internalpart and TEXT

Format:

print

Description:

Chloroplast polypeptide composition of rice plants {Oryza sativa L. cv. Safari) exposed for 30 d to increasing Cu ion concentrations in a hydroponie growth medium was analyised. The conventional discontinuous SDS-aerylamide gel electrophoretic systém, the SDS PhastGel (Pharmacia Fine Chemicals) gradient 10-15 and tíie 5-8 pH range polyaciylamide isoelectric focusing of the PhastSystem separation and development technique were ušed. With Cu levels greater than 0.25 g m'^ die polypeptide bands with apparent molecular masses of 42/41, 33/32, 21/20 and 19/18 kDa deereased in the PhastSystem separation and development systém, whereas with the conventional discontinuous SDS buffer systém gel electrophoresis all these bands disappeared except the 33/32 kDa band. Also, under the latter systém excess Cu deereased the 56/55, 55/54, 26 and 22 kDa bands which was not shown by the PhastSystem separation and development technique. Furthermore, in the discontinuous SDS-PAGE high Cu levels induced the disappearance of the 16.5, 14.5 and 12 kDa bands, which were again not shown by the PhastSystem separation and development technique; yet the opposite oceurred with the 49 kDa band of the gel profiles. Polyacrylamide isoelectric focusing of thyiakoid membranes showed in all Cu treatments two major bands at pl 7.7 and <5 and a minor one at pl 6.48. At Cu concentrations lower than 1.25 g m'^ two additional smáli bands appeared (pl 5.5 and 5.18); and at Cu concentrations greater than 0.25 g m'^ these smáli bands were replaced by four different ones (pl 6.7, 6.05, 5.35, 5.25).

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

109-117 and 127-135

Source:

Photosynthetica | 1993 Volume:28 | Number:1

Harvested from:

CDK

Metadata only:

false