Light distribution in leaf chambers and its consequences for photosynthesis measurements

Title:

Light distribution in leaf chambers and its consequences for photosynthesis measurements

Creator:

Hogewoning, S. W., Trouwborst, G., Harbinson, J., and van Ieperen, W.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:6c6f2e2f-a904-4dd0-b5a4-90599d382b49
uuid:6c6f2e2f-a904-4dd0-b5a4-90599d382b49
issn:0300-3604
doi:10.1007/s11099-010-0027-2

Subject:

botanika, botany, gas exchange, heterogeneity, leaf chamber, light distribution, photosynthesis, and photosynthetic quantum yield

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The impact of a heterogeneous distribution of actinic light within a leaf chamber for photosynthetic measurements by gas exchange on the photosynthesis-irradiance relationship was investigated. High-resolution light distributions were measured over the area of a commercially available clamp-on leaf chamber equipped with build-in red and blue LEDs, as well as over the area of a custom-made leaf chamber with external light source, using a low-cost digital camera and freely available software. The impact of the measured heterogeneity on the photosynthesis-irradiance response curve was calculated for two realistic scenarios. When the average light intensity over the leaf chamber area was estimated accurately, heterogeneity had minor effects on the photosynthesis-irradiance response curve. However, when the irradiance was measured in the chamber centre, which is common practice, and assumed to be homogeneous, for both leaf chambers the photosynthesis-irradiance response curve was subject to considerable error and led to serious underestimation of the light-limited quantum yield of photosynthesis. Additionally, mixed light sources with different heterogeneity patterns per light source, such as in the clamp-on leaf chamber, potentially increase errors due to heterogeneous physiological responses to light spectrum. High-resolution quantification of the leaf-chamber light distribution enables calculation of the correct average light intensity and already resolves the most pressing problems associated with heterogeneity. To exclude any light-distribution related errors in gas-exchange measurements a leaf chamber and actinic irradiance source design with a homogeneous light distribution is an absolute requirement. and S. W. Hogewoning ...[et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

219-226

Source:

Photosynthetica | 2010 Volume:48 | Number:2

Harvested from:

CDK

Metadata only:

false