UV-B induced production of MMP-2 and MMP-9 in human corneal cells

Title:

UV-B induced production of MMP-2 and MMP-9 in human corneal cells

Creator:

Igor Kozák, Klisenbauer, D., and Tomáš Juhás

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:6a92a364-c22a-4496-83ae-895b7ef97d73
uuid:6a92a364-c22a-4496-83ae-895b7ef97d73
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, UV-B irradiation, human corneal epithelial cells, human corneal fibroblasts, MMP-2 production, MMP-9 production, acute photokeratitis, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The purpose of this study was to determine the production of metalloproteinases (MMP) 2 and 9 following UV-B irradiation in human corneal epithelial cells and fibroblasts. Epithelial cells and fibroblasts were separated from human donor corneas and exposed to UV-B lamp irradiation for 20, 40, 80 and 120 s. Media samples were collected at 8, 24, 48 and 72 h and gelatinase A and B production was assayed by the ELISA test. Statistical significance of production was assessed by the paired t-test. Increased production of MMP-2 was found in human corneal fibroblasts in response to UV-B irradiation. A statistically significant production of MMP-2 was not observed in human corneal epithelial cells following UV-B exposure. We did not detect any increase in MMP-9 after irradiation in either epithelial cells or fibroblasts. MMP-2 is produced by the corneal fibroblasts in the acute phase after UV-B irradiation. MMP-9 is not released in vitro following UV-B irradiation damage and therefore does not directly participate in the pathophysiology of acute photokeratitis., I. Kozák, D. Klisenbauer, T. Juhás., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2003 Volume:52 | Number:2

Harvested from:

CDK

Metadata only:

false