Molecular imaging of brain lipid environment of lymphocytes in amyotrophic lateral sclerosis using magnetic resonance imaging and SECARS microscopy

Title:

Molecular imaging of brain lipid environment of lymphocytes in amyotrophic lateral sclerosis using magnetic resonance imaging and SECARS microscopy

Creator:

Machtoub, L., Bataveljić, D., and Andjus, P. R.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:652a1375-4233-4677-a1c5-097ec18540aa
uuid:652a1375-4233-4677-a1c5-097ec18540aa

Subject:

Fyziologie člověka a srovnávací fyziologie, nanočástice, neurodegenerace, lipidy, nanoparticles, neurodegeneration, lipids, USPIO, ALS, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

This paper highlights some of the key technologies of using two innovative molecular imaging modalites, magnetic resonance imaging (MRI) and nonlinear optical microscopy, for imaging intravenously injected ultra small paramagnetic iron oxide nanoparticles cross linked with antibodies (CLUSPIO) in the amyotrophic lateral sclerosi s (ALS) experimental model in vivo or ex vivo , respectively. Intensive efforts have been made in investigating the causes of abnormalities in lipid metabolism, monitored in some neurodegenerat ive disorders systems. It has been shown that an abnormal accumulation of some common lipids in motor nerve cells may play a critical role in the development of amyotrophic lateral sclerosis. The presented experiments were performed on brain specimens from the transgenic rat model expressing multiple copies of mutated (G93A) human SOD-1 gene, after CD4+ lymphocytes were magnetically labeled with i.v.i. CLUSPIO antibodies. In vivo MRI revealed marked signal intens ity enhancements in specific pathological regions of the ALS rat brain as compared to the wild type. Surface-enhanced coherent anti-Stokes Raman scattering (SECARS) microscopy indicated cellular interactions based on lipids association to anti-CD4 CLUSPIO., L. Machtoub, D. Bataveljić, P. R. Andjus., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:Suppl 1

Harvested from:

CDK

Metadata only:

false