Distribution of heterochromatin and rDNA on the holocentric chromosomes of the aphids Dysaphis plantaginea and Melanaphis pyraria (Hemiptera: Aphididae)

Title:

Distribution of heterochromatin and rDNA on the holocentric chromosomes of the aphids Dysaphis plantaginea and Melanaphis pyraria (Hemiptera: Aphididae)

Creator:

Criniti, Angela , Simonazzi, Gabriele, Cassanelli, Stefano, Ferrari, Mario, Bizzaro, Davide, and Manicardi, Gian Carlo

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:60b45151-1bc2-4b3b-9a31-c74eadf0fdbf
uuid:60b45151-1bc2-4b3b-9a31-c74eadf0fdbf

Subject:

Aphid, Cytogenetics, FISH, Holocentric chromosomes, Heterochromatin, and rDNA size heteromorphism

Type:

article, model:article, and TEXT

Description:

The structure of the holocentric chromosomes of the rosy apple aphid, Dysaphis plantaginea (2n = 12), and pear-grass aphid, Melanaphis pyraria (2n = 8), was studied using C-banding, NOR, Giemsa and fluorochrome staining, and fluorescent in situ hybridization (FISH). Contrary to the equilocal distribution of heterochromatin typical of monocentric chromosomes, in both species C-banding evidenced a tendency of highly repetitive DNAs to be restricted to the X chromosomes. Silver staining and FISH, using a 28S rDNA probe, located rDNA genes on one telomere of each X chromosome, the only brightly fluorescent C-positive sites revealed by CMA3 staining, whereas all other heterochromatic C-bands were DAPI positive. Both species showed a noticeable amount of rDNA heteromorphism. Mitotic recombination is proposed as a possible mechanism responsible for the variation in size of rDNA.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2009 Volume:106 | Number:2

Harvested from:

CDK

Metadata only:

false