Electroencephalogram is activated by addition of pentobarbital in the isolated perfused rat head
- Title:
- Electroencephalogram is activated by addition of pentobarbital in the isolated perfused rat head
- Creator:
- Tagawa, A., Mokuda, O., Sakamoto, Y., and Shimizu, N.
- Identifier:
- https://cdk.lib.cas.cz/client/handle/uuid:5f8f9db4-1155-4d74-a042-9b52da4eb0b1
uuid:5f8f9db4-1155-4d74-a042-9b52da4eb0b1
issn:0862-8408 - Subject:
- Fyziologie člověka a srovnávací fyziologie, elektroencefalogramy, electroencephalograms, in vitro, isolated brain perfusion, pentobarbital, 14, and 612
- Type:
- article, články, model:article, and TEXT
- Format:
- print, bez média, and svazek
- Description:
- To evaluate the direct effects of a barbiturate on cerebral functions without its influence on brain perfusion pressure, circulatory hormones and metabolites, the electroencephalogram (EEG) was studied in the isolated rat head. Male Wistar rats were anesthetized, and EEG electrodes were inserted into the cranium. A Krebs-Ringer bicarbonate buffer solution containing heparinized rat whole blood, 20 mmol/l glucose, 200 mmol/l mannitol and 0.1 mg/ml dexamethasone was used for the perfusate. The bilateral common carotid arteries were cannulated, pumped at a rate of 6 ml/min and the head was isolated. The venous effluent was reoxygenated and recirculated into the brain. Twenty-five min after isolation of the heads pentobarbital was added to the perfusate at concentrations of 0.1, 0.5 and 2.5 mg/ml. EEG was recorded before and during perfusion. EEG activity could be recorded for more than 25 min after the beginning of perfusion. EEG activity gradually declined from 42±5 μV before perfusion (in vivo) to 4±1 μV at 25 min after the beginning of perfusion. Then, 3 min after the addition of pentobarbital, the EEG activity became significantly higher in the high dose groups; 12±3 μV in the 0.5 mg/ml group (p<0.05) and 12±1 μV in 2.5 mg/ml group (p<0.05) compared with the group without pentobarbital (2±2 μV). The present study suggests that a barbiturate has mitigating effects on the brain damage induced by the in vitro brain perfusion., A. Tagawa, O. Mokuda, Y. Sakamoto, N. Shimizu., and Obsahuje bibliografii
- Language:
- English
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public - Source:
- Physiological research | 2003 Volume:52 | Number:4
- Harvested from:
- CDK
- Metadata only:
- false
The item or associated files might be "in copyright"; review the provided rights metadata:
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- policy:public