Differences in haemolytic action of Hg2+ in relation to its concentration and ionic strength of incubating solutions

Title:

Differences in haemolytic action of Hg2+ in relation to its concentration and ionic strength of incubating solutions

Creator:

Mojžiš, J. and Nicák, A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:5e73d1e2-4755-4384-bc05-b92a9550cf5d
uuid:5e73d1e2-4755-4384-bc05-b92a9550cf5d

Subject:

erythrocytes, HgCl2, haemolysis, and ionic strength

Type:

article, model:article, and TEXT

Description:

The haemolytic action of different concentrations of HgCl2 on rat red blood cells (RBC ) was studied in vitro. The concentrations of HgCb in incubating media were 0.15, 0.25 and 0.50 mmol.l'1. The ionic strength of the media varied from 0 to 154 mmol.l1 NaCl, Isotonicity of solutions was also compensated using isotonic glucose in different concentrations (287-0 mmol.l"1). Osmolarity of solutions varied from 287 to 308 mOsm. Besides these solutions the haemolysis in Krebs-Ringer solution was also studied. Haemolysis was characterized with two maxima in all concentrations of Hg2+. The first maximum was observed at low ionic strength and the second one at high ionic strength. In relation to the increased concentrations of Hg2+, the first maximum of haemolysis progressively declined towards the higher ionic strength. In the Krebs-Ringer solutions, the increased concentration of Hg2+ was followed by reduced haemolysis. The haemolytic concentration of 0.15 mmol.l"1 was found to be optimal.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1993 Volume:42 | Number:3

Harvested from:

CDK

Metadata only:

false