Linear dichroism, fluorescence polarization, and path of the thermal deactivation of excited cyanobacterial (Synechococcus elongatus) photosystem 1 immobilized and oriented in polymer films

Title:

Linear dichroism, fluorescence polarization, and path of the thermal deactivation of excited cyanobacterial (Synechococcus elongatus) photosystem 1 immobilized and oriented in polymer films

Creator:

Białek-Bylka, G. E., Sofrová, D., Szurkowski, J., Skwarek, R., Sopko, B., and Manikowski, H.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:5c595906-c6bf-4981-b07c-09a5c886b676
uuid:5c595906-c6bf-4981-b07c-09a5c886b676
issn:0300-3604
doi:10.1023/A:1026764412164

Subject:

carotenoids, photoacoustic and emission spectroscopy, polarized absorption, and reaction centres

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Pigment-protein complexes enriched in photosystem 1 (PS1) and, for comparison, enriched in photosystem 2 (PS2) were isolated from the cyanobacterium Synechococcus elongatus Nag. f. thermalis Geitl. They were immobilized and oriented in the polyvinyl alcohol (PVA) films, and studied by linear dichroism (LD), fluorescence polarization (FP), photoacoustic spectroscopy (PAS), and polarized photoacoustic spectroscopy (PAS|| and PAS⊥). The LD signal of β-carotene in the region with maximum at 500 nm was positive in the PS1 complex. The maximum value of fluorescence polarization (FP) in the measured photosynthetic pigment region was 1.25 and was similar to higher plant values. Carotenoids exhibited different efficiencies of thermal deactivation (max. at 500 nm) in PS1 and PS2. The thermal deactivation efficiency of carotenoids in comparison with that of chlorophyll (Chl) a at its red absorbance maximum was much higher in PS1 than in PS2 complexes. Cyanobacterial complexes did not contain Chl b, interpretation of the LD, PAS, and FP results is thus easier and can be compared with PS1 and PS2 values of higher plants, especially with Chl b-less mutant values. and G. E. Białek-Bylka ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

143-148

Source:

Photosynthetica | 2000 Volume:38 | Number:1

Harvested from:

CDK

Metadata only:

false