The influence of ascorbic acid on the hepatic cytochrome P-450, and glutathione in guinea-pigs exposed to 2,4-dichlorophenol

Title:

The influence of ascorbic acid on the hepatic cytochrome P-450, and glutathione in guinea-pigs exposed to 2,4-dichlorophenol

Creator:

Nagyová, A. and Ginter, E.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:57528ee5-d674-4c0f-8fd8-ac4a45ffad93
uuid:57528ee5-d674-4c0f-8fd8-ac4a45ffad93

Subject:

cytochrome P-450, ascorbic acid, glutathione, guinea-pig liver, and 2,4-dichlorophenol

Type:

article, model:article, and TEXT

Description:

Chlorophenols, mainly used as biocides, are compounds with a wide spectrum of toxic effects including teratogenic and carcinogenic actions. In this study, the effects of 2,4-dichlorophenol (2,4-DCP) on hepatic microsomal cytochrome P-450, NADPH-cytochrome c reductase activity, liver ascorbic acid (AA) and glutathione (GSH) content were studied in guinea-pigs with a low (2 mg/day/animal) or a high (50 mg/day/animal) ascorbic acid intake. The high AA intake significantly increased liver AA and GSH levels. There was a clear-cut correlation between liver AA and GSH levels. Administration of 2,4-DCP significantly decreased cytochrome P-450 and f iADPH-cytochrome c reductase activity in hepatic microsomes isolated from guinea-pigs with the low AA intake. Such a reduction was not observed in intoxicated guinea-pigs with the high AA intake. The results suggest that AA can play a protective role in 2,4-DCP toxicity.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1995 Volume:44 | Number:5

Harvested from:

CDK

Metadata only:

false