Time-lapse cleavage rating predicts human embryo viability

Title:

Time-lapse cleavage rating predicts human embryo viability

Creator:

Daniel Hlinka, Kal'atová, B., Uhrinová, I., Dolinská, S., Rutarová, J., Jitka Řezáčová, Sonja Lazarovská, and Dudáš, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:505f2606-29fc-429e-8d84-c4e2007327c7
uuid:505f2606-29fc-429e-8d84-c4e2007327c7

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, time-lapse imaging in human embryos, cell cycle analysis, trichotomic / Tripolar mitosis, selection for single-embryo transfer, preimplantation screening and diagnosis, clinical cytometry and morphometry, 14, and 612

Type:

article, články, model:article, and TEXT

Description:

Chronology of three consecutive mitotic events in human preimplantation embryos was examined by time-lapse imaging. In zygotes producing well-formed and pregnancy-yielding expanded blastocysts, uniform time-patterning of cleavage clusters (c) and interphases (i) was revealed: i2=11±1, i3=15±1, i4=23±1 h / c2=15±5, c3=40±10, c4=55±15 min. Oppositely, shortened or prolonged durations of one or more cell cycles were strongly predictive of poor implantation and development. Furthermore, trichotomic mitosis was discovered in 17 % of cases - zygotes cleaved into 3 blastomeres and 2-cell embryos into 5-6 cells (instead of normal 2 and 4). During conventional clinical assessment, such embryos are indistinguishable from normal, often considered just-in-course of the next cell cycle. Only detailed time-lapse monitoring paced at 10-minute intervals had proven all these embryos to be absolutely unviable, even in rare cases when they reduced their hypercellularity to normal cell counts via cell-cell fusion. Overall, we demonstrate that timelapse embryo cleavage rating (ECR) as a standalone diagnostic procedure allows for effective identification of viable early embryos with 90 % specificity, while elimination of good-looking but unviable embryos can be assumed with a specificity of 100 %. Thus, making this non-invasive and contactless approach worth of addition to routine embryo screening in clinical IVF programs., D. Hlinka ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2012 Volume:61 | Number:5

Harvested from:

CDK

Metadata only:

false