C-phycocyanin suppresses cell proliferation and promotes apoptosis by regulating the AMPK pathway in NCL-H292 non-small cell lung cancer cells

Title:

C-phycocyanin suppresses cell proliferation and promotes apoptosis by regulating the AMPK pathway in NCL-H292 non-small cell lung cancer cells

Creator:

Chaowen, He, Dongxuan, H., Dongsheng, H., Jianfeng, P., Fan, Y., Yahui, C., and Xiaohua, L.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:4f89fef2-bc0d-418b-893c-91cd378537d6
uuid:4f89fef2-bc0d-418b-893c-91cd378537d6

Subject:

AMPK, C-phycocyanin, mTOR, NSCLC, and NBR2

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Non-small cell lung cancer (NSCLC) results in high mortality and has gained increasing attention. C-Phycocyanin (C-PC) has been identified as a potential therapeutic inhibitor for NSCLC, but its underlying mechanism remains obscure. The gene expression of the long noncoding RNA neighbour of BRCAI RNA 2 (NBR2) in NSCLC cells was evaluated by quantitative reverse transcription-PCR. The cell capacity for proliferation and migration was examined by EdU and wound-healing assays. Furthermore, the viability and apoptosis of cells was measured with CCK-8 and annexin V/PI, respectively. Next, the protein level of activation of adenosine monophosphate- activated protein kinase and the rapamycin kinase (mTOR) signalling pathway-associated molecules was evaluated by western blotting. H292 cells were pre-treated with C-PC or transfected with plasmids encoding NBR2 or the shNBR2 plasmid, to over-express or knock down NBR2 expression, respectively. NBR2 expression was robustly down-regulated in NSCLC cell lines compared with a normal cell line (BEAS-2B). NBR2 over-expression inhibited migration and promoted apoptosis of H292 cells. Treatment of H292 cells with C-PC enhanced NBR2 levels in a dose- and time-dependent manner. Downregulation of NBR2 in H292 cells inhibited the activity of C-PC on cell proliferation, viability and clone formation. Further mechanistic investigation showed that the down-regulation of NBR2 abolished the modulatory effects of C-PC on the AMPK/mTOR signalling pathway. In conclusion, C-PC inhibits H292 cell growth by enhancing the NBR2/AMPK signalling pathway.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

16-24

Source:

Folia biologica | 2022 Volume:68 | Number:1

Harvested from:

CDK

Metadata only:

false